GLAST (ACSA-1) Antibody, anti-human/mouse/rat, PE 130-118-344 from Miltenyi Biotec

Product Specs
ItemGLAST (ACSA-1) Antibody, anti-human/mouse/rat, PE
CompanyMiltenyi Biotec
Price Pricing Info Supplier Page View Company Product Page
Catalog Number130-118-344
Quantity100 tests in 200 µL
ApplicationsFlow cytometry, MICS (MACSima Imaging Cyclic Staining), Immunofluorescence, Immunohistochemistry
Conjugate/TagPE
Reactivityhuman, mouse, rat
IsotypeIgG2a
CloneACSA-1
Hostmouse
Antibody TypePrimary antibodies
Clonalitymonoclonal
Concentration1:50
FormatReagents are supplied in buffer containing stabilizer and 0.05% sodium azide
TargetGLAST (ACSA-1)
Research AreaFor research use only
Regulatory StatusFor research use only

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Miltenyi Biotec

Miltenyi Biotec Inc.

2303 Lindbergh Street

Auburn, CA 95602

United States

Phone: Phone: +1 530 888 8871
Toll Free: 800 FOR MACS
Fax: +1 530 888 8925

Email: macs@miltenyibiotec.com

Company Profile

Website: www.miltenyibiotec.com

International Office

Miltenyi Biotec

Miltenyi Biotec B.V. & Co. KG

Friedrich-Ebert-Straße 68

51429 Bergisch Gladbach

Germany

Phone: +49 2204 8306-0
Fax: +49 2204 8306-235

Email: macs@miltenyibiotec.de

Company Profile

Website: www.miltenyibiotec.com

(1) Autophagy drives the conversion of developmental neural stem cells to the adult quiescent stateNature CommunicationsNovember 24, 2023Isabel Calatayud-Baselga, Lucía Casares-Crespo, Carmina Franch-Ibáñez, José Guijarro-Nuez, Pascual Sanz, Helena Miraanalysis. For hippocampal NSCs isolation, cells were labeled with GLAST (ACSA-1)-PE (1:50, Miltenyi, 130-118-483) and Anti-Prominin-1-APC (1:10, Miltenyi, 130-102-197) antibodies and were isolated by FACS (BD FACSAria™

(2) High-throughput single-cell analysis reveals progressive mitochondrial DNA mosaicism throughout lifeScience AdvancesOctober 27, 2023Angelos Glynos, Lyuba V. Bozhilova, Michele Frison, Stephen Burr, James B. Stewart, Patrick F. Chinnery(PE)–Vio770 [Miltenyi Biotech, product number (PN): 130-102-153] and GLAST (ACSA-1)–PE (Miltenyi Biotech, PN: 130-118-344), while adult mouse brain samples were stained with GLAST (ACSA-1)–PE (Miltenyi Biotech, PN: 130-118-344)

(3) In Vitro Functional Characterization of Type-I Taste Bud Cells as Monocytes/Macrophages-like Which Secrete Proinflammatory CytokinesInternational journal of molecular sciencesJune 19, 2023Hichami A, Saidi H, Khan AS, Degbeni P, Khan NA.molecular expression was monitored using the following fluorochrome-labeled antibodies: anti-GLAST PE (ref 130-118-344) and anti-CD11b FITC (ref 130-113-234) diluted at 1:50; anti-CD64 APC diluted at 1:20 and anti-F4/80

(4) Association between inflammation, reward processing, and ibuprofen-induced increases of miR-23b in astrocyte-enriched extracellular vesicles: A randomized, placebo-controlled, double-blind, exploratory trial in healthy individualsBrain, Behavior, & Immunity - HealthFebruary 1, 2023Kaiping Burrows, Leandra K. Figueroa-Hall, Ahlam M. Alarbi, Jennifer L. Stewart, Rayus Kuplicki, Chibing Tan, Bethany N. Hannafon, Rajagopal Ramesh, Jonathan Savitz, Sahib Khalsa, et al.fluorescent tag that specifically binds to the astrocyte surface marker, GLAST-PE (Miltenyi Biotec Inc; Cat #130-118-483); and (b) subsequently analyzed by flow cytometry to confirm AEEV capture. The bead/antibody/EV complex

(5) Early Permissiveness of Central Nervous System Cells to Measles Virus Infection Is Determined by Hyperfusogenicity and Interferon PressureVirusesJanuary 13, 2023Ferren M, Lalande A, Iampietro M, Canus L, Decimo D, Gerlier D, Porotto M, Mathieu C.594,Merk/sigma; SAB5202234-100UG,1/100 Astrocytes,"Anti GLAST (ACSA-1), anti-human/mouse/rat",PE,Miltenyi; 130-118-344,1/10 Oligodendrocytes,Clone mGalC Anti-Galactocerebroside,Alexa fluor 647,Merk/sigma; MAB342-AF647,1/100 Microglial cells

(6) Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analysesSTAR protocolsJune 17, 2022Milner MT, Lawrence GM, Holley CL, Bodea LG, Götz J, Burgener SS, Schroder K.(Becton, Dickinson & Company)",Cat.#564997; RRID: AB_2869637 GLAST PE (1:75),Miltenyi Biotec,"Clone: ACSA-1, Cat.#130-118-344; RRID: AB_2733723" CD16/32 (clone 2.4G2) (1:100),BD Bioscience (Becton Dickinson & Company),"Clone:

(7) Glial receptor PLXNB2 regulates schizophrenia-related stress perception via the amygdalaFrontiers in immunologyJanuary 1, 2022Xuan FL, Yan L, Li Y, Fan F, Deng H, Gou M, Chithanathan K, Heinla I, Yuan L, Seppa K, et al.(#130-123-555) were used. For microinjection experiment, CD11b-FITC (#101206), CD45-PE/Cy7 (#103114), Glast-PE (#130-118-344, Miltenyi), and MHCII-BV711 (#107643) were used. Washed samples were finally resuspended with FC buffer

(8) Flow cytometry analysis of immune and glial cells in a trigeminal neuralgia rat modelScientific reportsDecember 7, 2021Lin J, Zhou L, Luo Z, Adam MI, Zhao L, Wang F, Luo D.flow cytometry antibodies CD45-PE-Cy7 and CD11b-V450 (561588 and 562108, BD Pharmingen, NJ, USA), GLAST (130-118-344, Miltenyi Biotec, Bergisch Gladbach, Germany) and incubating at room temperature for 15 min in the dark.

(9) A ketogenic diet differentially affects neuron and astrocyte transcriptionJournal of NeurochemistryJune 1, 2021Scott J. Koppel et al.labeling. The stains/antibodies were as follows: GLAST-PE antibody for astrocytes (Miltenyi Biotec cat no. #130-118-344, RRID: AB_2733722), CD11b-PE/Cy7 antibody (Miltenyi Biotec cat no. #101216) for microglia, O4-APC (Miltenyi Biotec

(10) βA1-crystallin regulates glucose metabolism and mitochondrial function in mouse retinal astrocytes by modulating PTP1B activityCommunications biologyFebruary 24, 2021Ghosh S, Liu H, Yazdankhah M, Stepicheva N, Shang P, Vaidya T, Hose S, Gupta U, Calderon MJ, Hu MW, et al.ICRF44, BD Biosciences, USA; Cat# 563088) and GLAST1 (PE, clone ASCA-1, Miltenyi Biotec, USA; Cat# 130-118-344). Data acquisition was done on a flow cytometer (BD FACS Lyric, BD Biosciences, USA) and analysed using

(11) Nitric Oxide Induces a Janus Kinase-1-Dependent Inflammatory Response in Primary Murine AstrocytesASN neuroJanuary 1, 2021Nowery JD, Cisney RN, Feldmann JW, Meares GP.plates. Astrocytes are enriched to ∼90% GLAST1 + cells as determined by flow cytometry (Miltenyi Biotec# 130-118-483, RRID:AB_2733722). Experimental treatments were initiated 48–72 h after plating. Genotyping and Cre

(12) Dissociation of neonatal and adult mice brain for simultaneous analysis of microglia, astrocytes and infiltrating lymphocytes by flow cytometryIBRO reportsJune 1, 2020Calvo B, Rubio F, Fernández M, Tranque P.(reference) ACSA-1-PE (anti-GLAST),ACSA-1,Neonatal astrocytes and radial glia,1:25,Miltenyi Biotec (130-118-483) ACSA-2-APC,IH3-18A3,Neonatal and adult astrocytes,1:25,Miltenyi Biotec (130-117-535) FcR Blocking

(13) Deletion of calcineurin from GFAP‐expressing astrocytes impairs excitability of cerebellar and hippocampal neurons through astroglial Na⁺/K⁺ATPaseGliaMarch 1, 2020Laura Tapella et al.μM, Thermo Fisher, Milan, Italy, Cat. 62251), anti GLAST(ACSA-1) PE-conjugated antibody (1:250, Cat 130-118-483) and with anti-CD90.2 FITCconjugated antibody (Cat. 130-102-452) (last two from Miltenyi Biotech, Bologna,

(14) Prospective identification of functionally distinct stem cells and neurosphere-initiating cells in adult mouse forebraineLifeMay 7, 2014Mich JK, Signer RA, Nakada D, Pineda A, Burgess RJ, Vue TY, Johnson JE, Morrison SJ.

(15) Translation of the prion protein mRNA is robust in astrocytes but does not amplify during reactive astrocytosis in the mouse brainPloS oneJanuary 1, 2014Jackson WS, Krost C, Borkowski AW, Kaczmarczyk L.

(16) Recurrent somatic mutation of FAT1 in multiple human cancers leads to aberrant Wnt activationNature geneticsMarch 1, 2013Morris LG, Kaufman AM, Gong Y, Ramaswami D, Walsh LA, Turcan Ş, Eng S, Kannan K, Zou Y, Peng L, et al.

(17) The problem of astrocyte identityNeurochemistry internationalJuly 1, 2004Kimelberg HK.

(18) Radial glia diversity: a matter of cell fateGliaJuly 1, 2003Kriegstein AR, Götz M.

(19) Membrane topology of the high-affinity L-glutamate transporter (GLAST-1) of the central nervous systemThe Journal of cell biologyDecember 1, 1996Wahle S, Stoffel W.

(20) Structure, expression, and functional analysis of a Na(+)-dependent glutamate/aspartate transporter from rat brainProceedings of the National Academy of Sciences of the United States of AmericaNovember 15, 1992Storck T, Schulte S, Hofmann K, Stoffel W.

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GLAST (ACSA-1) Antibody, anti-human/mouse/rat, PE from Miltenyi Biotec

Miltenyi Biotec

Miltenyi Biotec

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GLAST (ACSA-1) Antibody, anti-human/mouse/rat, PE from Miltenyi Biotec

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